Occurrence of hyaluronidase inhibitors in fractions of electrophoretically separated serum.

An inhibitor to the action of hyaluronidase obtained from bovine testis has recently been described in human sera, and has been found to be associated with electrophoretically separated albumin (1). Another inhibitor of hyaluronidase has been described recently which inhibits streptococcal hyaluronidase and which gives evidence of being a true neutralizing antibody (24). Because of differences suggested between the natures of these two inhibitors by the studies referred to, the present study was undertaken to compare the antihyaluronidase activity found in electrophoretically separated samples of human serum when assayed with streptococcal and testicular hyaluronidase.